Pedro Magalhães (ESR9 in Mosaiques Diagnostics) has recently collaborated in 2 publications: “Urinary proteomics using capillary electrophoresis coupled to mass spectrometry for diagnosis and prognosis in kidney diseases” (Pedro Magalhães, Harald Mischak and Petra Zürbig) and “Urinary biomarkers for renal tract malformations” (Pedro Magalhães, Joost P. Schanstra, Emma Carrick, Harald Mischak and Petra Zürbig).
Current Opinion in Nephrology & Hypertension: November 2016 – Volume 25 – Issue 6 – pages 494–501
Urinary proteomics using capillary electrophoresis coupled to mass spectrometry for diagnosis and prognosis in kidney diseases
Pedro Magalhães, Harald Mischak and Petra Zürbig
Purpose of review: Urine is the most useful of body fluids for biomarker research. Therefore, we have focused on urinary proteomics, using capillary electrophoresis coupled to mass spectrometry, to investigate kidney diseases in recent years.
Recent findings: Several urinary proteomics studies for the detection of various kidney diseases have indicated the potential of this approach aimed at diagnostic and prognostic assessment. Urinary protein biomarkers such as collagen fragments, serum albumin, α-1-antitrypsin, and uromodulin can help to explain the processes involved during disease progression.
Summary: Urinary proteomics has been used in several studies in order to identify and validate biomarkers associated with different kidney diseases. These biomarkers, with improved sensitivity and specificity when compared with the current gold standards, provide a significant alternative for diagnosis and prognosis, as well as improving clinical decision-making.
Keywords: biomarkers, capillary electrophoresis-mass spectrometry, kidney diseases, urinary proteomics
Expert Review of Proteomics: 2016 – Volume 13 – Issue 12 – Pages 1121-1129
Urinary biomarkers for renal tract malformations
Pedro Magalhães, Joost P. Schanstra, Emma Carrick, Harald Mischak and Petra Zürbig
Introduction: Renal tract malformations (RTMs) are congenital anomalies of the kidneys and urinary tract, which are the major cause of end-stage renal disease in children. Using immunoassay-based approaches (ELISA, western blot), individual urinary proteins including transforming growth factor β, tumor necrosis factor and monocyte attractant proteins 1 were found to be associated to RTMs. However, only mass spectrometry (MS) based methods leading to the identification of panels of protein-based markers composed of fragments of the extracellular matrix allowed the prediction of progression of RTMs and its complications.
Areas covered: In this review, we summarized relevant studies identified in “Pubmed” using the keywords “urinary biomarkers” and “proteomics” and “renal tract malformations” or “hydronephrosis” or “ureteropelvic junction obstruction” or “posterior urethral valves” or “vesicoureteral reflux”. These publications represent studies on potential protein-based biomarkers, either individually or combined in panels, of RTMs in human and animal models.
Expert commentary: Successful use in the clinic of these protein-based biomarkers will need to involve larger scale studies to reach sufficient power. Improved performance will potentially come from combining immunoassay- and MS-based markers.
Keywords: proteomics; children; urine; biomarkers; renal tract malformation
3rd April 2017